DNA

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  • Created by: Ashlee
  • Created on: 04-05-15 19:00
Where can DNA be found?
White blood cells, semen and saliva
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How would you profile DNA?
Polymerase chain reaction and short tandem repeats
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What are polymerase chain reactions (PCR)?
Amplifying pieces of DNA across several magnitudes, generating a thousand/million copies of a DNA sequence
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What are short tandem repeats (STR's)?
Measures the exact number of repeating nucleotides of a microsatellite
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What is junk DNA?
DNA that doesn't code for anything/make proteins
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What is a phenotype?
Inherited physical appearance from dominant alleles
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What is a genotype?
Genetic makeup of a specific characteristic due to dominant and recessive alleles
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What is cell free DNA?
Touch DNA; transferred nucleated cells; free running nucleic acids in sweat; sloughed skin cells
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Where do you find DNA in a cell?
Nucleus
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How would you profile DNA?
DNA17; YSTR's; LCN; SGM+; DNA SenCE; MtDNA
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What is DNA17?
32 DNA components and gender indentifier; improved discrimination, sensitivity and comparability
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What is YSTR's?
Short tandem repeat on the Y chromosome
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Why would you use YSTR's?
Women have no Y chromosome; DNA of both man and women could mix during events; when dealing with sexual assaults
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What is LCN?
Low copy number
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Why would you use LCN?
Retrieve data from small or degraded samples; does 34 cycles which is more than the usual system
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What is SGM+?
Second generation multiplex plus - analyses 10 STR markers and gender indicators for 28 cycles
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What is DNA SenCE?
Enhancement from small samples; cleans and concentrates
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What is MtDNA?
Mitochondrial DNA passed down from the mother
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Other cards in this set

Card 2

Front

How would you profile DNA?

Back

Polymerase chain reaction and short tandem repeats

Card 3

Front

What are polymerase chain reactions (PCR)?

Back

Preview of the front of card 3

Card 4

Front

What are short tandem repeats (STR's)?

Back

Preview of the front of card 4

Card 5

Front

What is junk DNA?

Back

Preview of the front of card 5
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