DNA profiling

HideShow resource information
What does PCR stand for?
Polymerase Chain reaction
1 of 15
What is placed in the test tube for PCR?
free mononucleotides, DNA primers and DNA polymerase
2 of 15
What is the first temperature the solution is heated to?
95 degrees celsius
3 of 15
Why?
The DNA becomes denatured, H bonds break
4 of 15
What is the next temperature?
55 degrees celsius
5 of 15
What happens here?
small pieces of DNA with fluorescent markers, attach at the start of the STR repeated sequence
6 of 15
What is the next temperature?
heated to 70 degrees celsius
7 of 15
What attaches here?
DNA polymerases and nucleotides are added extending the DNA from the Primer
8 of 15
What is the first stage of DNA profiling?
Copy a sample of DNA using PCR
9 of 15
What happens to the DNA?
IT is cut using restriction enzymes- endonucleases
10 of 15
What happens to the sample of DNA?
Its run through an electrophoresis gel
11 of 15
What is the DNA attracted to?
the anode because it is negatively charged.
12 of 15
What is the DNA transferred to?
nylon or nictroclellulose membrane
13 of 15
Where is the membrane placed?
in a bag with DNA probe
14 of 15
Why is a DNA probe important?
It is radioactive.
15 of 15

Other cards in this set

Card 2

Front

What is placed in the test tube for PCR?

Back

free mononucleotides, DNA primers and DNA polymerase

Card 3

Front

What is the first temperature the solution is heated to?

Back

Preview of the front of card 3

Card 4

Front

Why?

Back

Preview of the front of card 4

Card 5

Front

What is the next temperature?

Back

Preview of the front of card 5
View more cards

Comments

No comments have yet been made

Similar Biology resources:

See all Biology resources »See all Forensics resources »