DNA Fingerprinting

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What is the first stage in DNA fingerprinting
collect a DNA sample
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What is the second stage in DNA fingerprinting
Fragment DNA with restriction enzymes
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What is the third stage in DNA fingerprinting
"load" fragments into a gel, run an electric current through the gel. DNA is negitive and will move to the positive end
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What is the fourth stage in DNA fingerprinting
smaller fragments move faster creating a band pattern on the gel, then you read the pattern
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whats the first step in engineering bacteria to produce insulin
identify the gene which controls the production of insulin
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Whats step 2
remove the DNA from the cell by cutting it out with a restriction enzyme. DNA is left with sticky ends
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Whats step 3
a loop of bacterial DNA (a plasmid) is cut open with the same restriction enzyme so it has sticky ends to
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what is step 4
the insulin is inserted in the plasmid, the sticky ends allow another enzyme (ligase) to join DNA strands together
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what are the four stages of infectious disease
1) micro-orgaism enters the body 2) reproduces rapidly 3) toxins are produced 4) toxins cause symptoms
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whats the first stage in viruses reproducing
virus attaches itself to a specific host cell where it injects its genetic material
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whats stage 2
virus then uses the host cell to make the components of a new virus
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whats stage 3
the host cell splits open releasing the new virus
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how do bacteria reproduce
binary fission
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Card 2

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What is the second stage in DNA fingerprinting

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Fragment DNA with restriction enzymes

Card 3

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What is the third stage in DNA fingerprinting

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Card 4

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What is the fourth stage in DNA fingerprinting

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Card 5

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whats the first step in engineering bacteria to produce insulin

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