DNA Extraction Experiment 0.0 / 5 ? BiologyDNA and inheritanceGCSEEdexcel Created by: katiebrosterCreated on: 15-04-16 12:08 what does putting the (e.g) onion in a detergent and salt solution (step 1) do? break down cell membranes and make DNA stick together 1 of 6 what does putting the beaker into the water bath (step 2) do? denatures enzymes that could digest the DNA and softens cells 2 of 6 what does putting the beaker into ice (step 3) do? cools the mixture and stops the DNA from breaking down 3 of 6 what does putting the mixture into a blender (step 4) do? breaks open the cells walls and releases the DNA 4 of 6 what does cooling the mixture again and filtering it (step 5)? gets rid of the froth and big bits of cell 5 of 6 what does gently adding ice-cold alcohol (step 6) do? DNA will start to come out of solution (not soluble in cold alcohol) which can be fished out with a glass rod 6 of 6
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