How is timing of DNA replicators firing coordinated?
By compaction of chromatin
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What is 'active' chromatin, and when does it replicate?
Loosely bound (euchromatin), replicates early in the S-phase
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What is 'inert' chromatin, and when does it replicate? Give example
Highly condensed (heterochromatin), replicates late e.g. X chromosome
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What are AT rich sequences associated with?
Late replication
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What is the role of replication licensing?
It maintains the correct gene copy number in daughter cells
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What does 'licensing' entail?
RLF putting replicators in an initiation competent state
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What do RLF and SPF stand for?
Replication Licensing Factor and S-phase Promoting Factor
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Where are they found?
RLF= cytoplasmic, SPF= nuclear
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What do they do?
RLF- licenses replicators (so they are in an invitation competent state) SPF= induces initiation and removes license
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Comment on the a) no. of subunits, b) proofreading capability, c) processivity d) role of 1) DNA pol alpha 2) DNA pol delta
1) a) 4 b) none c) low d) initiation of leading and lagging synthesis 2)a) 4-12 b) proofreading ability c) high processivity when associated with PCNA d) mainly leading strand synthesis
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What are the roles of a) B and E DNA polymerases b) gamma DNA polymerases
a) DNA repair b) replication of mitochondrial DNA
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Other cards in this set
Card 2
Front
What three essential DNA sequences are needed?
Back
1) replication origin 2)centromere 3) telomere
Card 3
Front
what is a centromere?
Back
Card 4
Front
Why do eukaryotes require multiple sites of origin? (2 reasons)
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