Biology-B1-Culturing Microorganisms
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- Created by: tonisha_
- Created on: 22-09-21 19:54
What is Binary Fission?
the dividing and multiplying of bacteria
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How often would this happen?
every 20 mins
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When will it happen every 20 mins?
when the bacteria is under optimum conditions
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What are some conditions needed for bacteria to grow?
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temperature
oxygen
glucose
proteins(nitrogen)
pH level
oxygen
glucose
proteins(nitrogen)
pH level
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What is a colony?
a group of bacteria visible to the naked eye
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What is one reason why people grow bacteria?
to find out what nutrients are needed for them to grow
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What is another reason why people grow bacteria?
investigate what chemicals are best for killing them
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What are some examples of things that can kill bacteria?
disinfectants
antibiotics
antibiotics
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What is the Asceptic technique?
a method that prevents contamination and is safe
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Why would someone prefer this technique over any other?
we may accidentally grow dangerous pathogens or they may become contaminated
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Growing Bacteria in a Lab
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What is the first thing you should do with the petri dish?
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What is the first thing you should do with the petri dish?
sterilise it
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What should you sterilise it with?
an autoclave
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What does this prevent and destroy?
contamination and destroys pathogens
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What two things can a culture medium be?
What does a culture medium include?
What does it allow the bacteria to do?
What does a culture medium include?
What does it allow the bacteria to do?
Can be a nutrient broth solution or agar jelly
includes essential nutrients for bacteria to grow
it allows bacteria to form visible colonies on surface
includes essential nutrients for bacteria to grow
it allows bacteria to form visible colonies on surface
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Why do we boil the agar jelly?
it sterilises it and prevents contamination and destroys pathogens
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What does they agar jelly contain?
nutrients needed for the bacteria to grow
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What do we then do with the inoculating loop?
sterilise it
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What do we sterilise it with?
a bunsen burner
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What does this destroy?
pathogens
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What else may we use to sterilise the inoculating loop?
ethanol
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What do we then do with the sterilised agar?
Then we allow it to ?
Then we allow it to ?
pour it into a petri dish
cool and set
cool and set
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Then how do we transfer the bacteria onto the petri dish?
using the inoculating loop
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What do we then do with the inoculating loop?
sterilise it again
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Why do we sterilise it again?
to kill any unwanted pathogens
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Why do we keep the petri dish near the flame when dealing with it?
so that any pathogens in the air or environment get destroyed
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What do we then place on the petri dish?
a lid
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Why do we place a lid?
to prevent contamination and keep any unwanted pathogens away
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Then what do we put on the sides of the petri dish?
tape
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Why do we only put tape on the sides?
to allow oxygen to enter
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What happens if the oxygen is unable to reach bacteria?
bacteria will respire anaerobically instead of aerobically
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Why is this bad?
this could grow a dangerous bacteria
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Then how do we place the petri dish ?
upside down
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Why do we place it upside down?
to avoid condensation
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Where do we then place the petri dish?
in an incubator
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At how many degrees?
25'C
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Why can it not be higher that 25'C?
that could grow a dangerous pathogen
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What is a disinfectant?
kills bacteria in the environment
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What is an antiseptic?
a disinfectant safe to use on the skin
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What is an antibiotic?
chemicals safe to use inside the body
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INVESTIGATE EFFECT OF ANTIBIOTICS ON BACTERIA
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Firstly, cover the surface of _____ _____ in an _____ layer of ______?
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Firstly, cover the surface of _____ _____ in an _____ layer of ______?
cover the surface of agar jelly in an even layer of bacteria
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Then use ______ discs?
paper
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Soaked in either of what two things?
different types of antibiotics
different conc of one type of antibiotic
different conc of one type of antibiotic
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Place the disc?
onto the jelly leaving some space between them
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The antibiotic should ______ into?
diffuse into the jelly
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If bacteria have died?
a clear area will be left?
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This is called?
the inhibition zone
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We must make sure to use a ?
control
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What would the control be?
a paper disc soaked in sterile water
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We leave the plate for how long and at what temp?
48 hours
25 degrees
25 degrees
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The more effective the antibiotic?
the bigger the inhibition zone
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How do we calculate are of inhibition zone?
area of circle
pi x radius squared
pi x radius squared
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Other cards in this set
Card 2
Front
How often would this happen?
Back
every 20 mins
Card 3
Front
When will it happen every 20 mins?
Back
Card 4
Front
What are some conditions needed for bacteria to grow?
(4-5)
(4-5)
Back
Card 5
Front
What is a colony?
Back
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