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1. Which purpose of the gel is correct?
- polyacrylamind gel - electrophoretic separation of nucleic acids
- Silica gel - chromatography, drying
- Agarose gel - electrophoretic separation of protiens
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2. which is not true?
- Thin layer chromatography - separates substances with different affinity to 2 phases (staionary and mobile phase)
- surfactants are compounds that increase surface tension (adhesive and cohesive forces)
- there are 4 types of surfactants - anion, cationic, amphoteric, non ionic
- when the monomer conc > CMC then a micelle is formed
3. Which of these will increase stabilization of a colloid particle?
- Adding the ionic strength by adding
- Adding water soluble organic solvent (destroys the lyosphere)
- Add a little salt (eg. increases solubility of globulins)
- Changing the Ph to its isoelectric point for amphoteric colloid particles like proteins
4. what is a protective colloid?
- A protective colloid are substances that form a hydrophilic colloid solution stabilizing the hydrophobic colloid particles.
- proteins in the blood are protective colloids of Calcium phosphate and calcium carbonate leading to its precipitation (forming gall stones)
- A protective colloid are substances that form a hydrophobic colloid solution stabilizing the hydrophillic colloid particles.
- A stubstance that specifically stabilises emulsions eg glycocholic (facilate absorption of dietary lipids)
5. which of these is not a feature of a colloid?
- Optical feature: Opalescent
- > 500nm
- settles by ultracentrifugation - cannot use filtration
- Allows dialysis
- Contributes to the oncotic pressure
- Because of their large size- they can induce light diffusion
- Can be seen by electric microscope
- Can be separated by electrophoresis because they often carry a charge
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