Colloids

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1. which of these is not a feature of a colloid?

  • Optical feature: Opalescent
  • Can be separated by electrophoresis because they often carry a charge
  • Contributes to the oncotic pressure
  • Because of their large size- they can induce light diffusion
  • > 500nm
  • Can be seen by electric microscope
  • settles by ultracentrifugation - cannot use filtration
  • Allows dialysis
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2. which is not true?

  • there are 4 types of surfactants - anion, cationic, amphoteric, non ionic
  • Thin layer chromatography - separates substances with different affinity to 2 phases (staionary and mobile phase)
  • surfactants are compounds that increase surface tension (adhesive and cohesive forces)
  • when the monomer conc > CMC then a micelle is formed

3. In the venule end which type of pressure is higher?

  • Blood pressure < Oncotic pressure so tissue fluid returns back into the cappillaries
  • Blood pressure > Oncotic pressure so tissue fluid leaves the cappillaries
  • Blood pressure > Oncotic pressure so tissue fluid returns back into the cappillaries
  • Blood pressure < Oncotic pressure so tissue fluid leaves the cappillaries

4. Which of these will increase stabilization of a colloid particle?

  • Adding the ionic strength by adding
  • Adding water soluble organic solvent (destroys the lyosphere)
  • Add a little salt (eg. increases solubility of globulins)
  • Changing the Ph to its isoelectric point for amphoteric colloid particles like proteins

5. Which purpose of the gel is correct?

  • Silica gel - chromatography, drying
  • Agarose gel - electrophoretic separation of protiens
  • polyacrylamind gel - electrophoretic separation of nucleic acids

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