Chrymotrypsin

What is chymotrypsin?
An enzyme for breaking peptide bonds. it has a large cavity and no preference of charge of protien
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Trypsin
large cavity only +ve residues
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elastase
Smaller cavity and prefers hydrophobic residues
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Experimental studies of chymotrypsin
uses esters which produce a colour to show enzyme kinetics showing burst and then slower phase.
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Defining it's mechanism
we can trap acyl-enzyme intermediate to make sure. by making the solution very acidic so that the acyl intermediate cannot be release by the nucleophilic attack of water.
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Other cards in this set

Card 2

Front

Trypsin

Back

large cavity only +ve residues

Card 3

Front

elastase

Back

Preview of the front of card 3

Card 4

Front

Experimental studies of chymotrypsin

Back

Preview of the front of card 4

Card 5

Front

Defining it's mechanism

Back

Preview of the front of card 5

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