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6. polar compounds in the mixture pass faster than none polar compounds

  • reverse phase hplc
  • normal phase hplc

7. decreasing N leads to

  • flatter chromatography bands
  • sharper chromatography bands

8. column efficiency

  • N
  • a
  • K'x
  • Rf

9. on a chromatogram, the area under the peak is proportional to the

  • amount of x which has passed the detector
  • the retention time

10. ____________ hplc is the most commonly used form

  • reversed
  • normal

11. the speed that the compounds travel in tlc depends on how solvent the compound is in the solvent and how much attraction is present between molecules and stationary phase

  • True
  • Fasle

12. if a = 1 there is

  • no selectivity
  • good selectivity

13. none polar solvant

  • normal phase hplc
  • reverse phase hplc

14. the mobile phase in thin layer chrom is a suitable liquid solvent or mixture of solvents

  • True
  • False

15. _____________ usually destroys the sample, the signal is related to the rate at which solute molecules enter the detector - unaffected by make up gas

  • mass flow detector
  • concentration dependant detector

16. ninhydirin is used for

  • showing tlc spots up chemically
  • fluorescense to show tlc spots up

17. __ = conc of solute on stationary phase/conc of solute in mobile phase

  • retention time Rf
  • distribution ration Kx
  • distribution ration Rf
  • Retention time Kx

18. retention time will vary for compounds due to pressures (flow rate), stationary phase, composition of the solvent, temperature of column

  • true
  • false

19. in ______ solvent is forced through a column under high pressures of up to 40 atm

  • HPLC
  • column chrom
  • thin layer chrom
  • paper chrom

20. ____ = distance travelled by component/distance travelled by solvent

  • Rf
  • Retardation factor