chromatography

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  • Created by: hollie
  • Created on: 14-11-14 15:02
What is chromatography?
chromatography separates analytes by their interaction with stationary phase, and for two compounds to separate they must interact with the stationary phase to a different extent.
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Describe and show the equation for the partition coefficient.
Analytes that have strong interactions with the stationary phase will travel slower than analytes with weak interactions. K=[Cs]/[Cm] C= concentration at stationary and mobile phases.
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Describe the stationary and mobile phase and the format for gas chromatography.
SP- immobilised film coated on small particles of silica or alumina. MP- inert gas. F- packed column or open capillary
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Describe the stationary and mobile phase and the format for liquid chromatography.
SP- cellulose fibres or partials with varying polarity surfaces. MP- inert liquid. F- paper sheet, large diameter packed column or small diameter packed column.
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Describe the stationary and mobile phase and the format for supercritical chromatography.
SP- can use either GC or HPLC columns. MP- liquid or gas under elevated temperatures and pressures. F- packed column or open capillary
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What is column chromatography?
Where the components in the sample are separated as they move through the stationary phase. The mobile phase carries the components through the column until they elute from the end of the column. The detector is used to detect and quantify
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What is planar chromatography?
The components separate as they elute along a planar layer, and the components remain on the separation media at the end
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What is the equation for adjusted retention time?
tr'= (tr-tm). tr'= adjusted retention time tr= retention time of analyte tm= retention time of an unretained compound
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What is the equation for capacity of retention factor?
K'= (tr-tm)/tm K'= capacity or retention factor tr= retention time of analyte tm=Retention time of unretained compound
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What is the equation for height equivalent to theoretical plate?
HETP= L/N HETP= height equivalent to a theoretical plate L=length of column N= number of theoretical plates
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What is the equation for number of theoretical plates?
N=16(tr^2/w^2) N= number of theoretical plates tr= retention time of analyte w=width of peak
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What is the equation for resolution?
R= (tr2-tr1)/0.5(w2+w1) R= resolution tr= retention time of analytes w=width of peak
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Other cards in this set

Card 2

Front

Describe and show the equation for the partition coefficient.

Back

Analytes that have strong interactions with the stationary phase will travel slower than analytes with weak interactions. K=[Cs]/[Cm] C= concentration at stationary and mobile phases.

Card 3

Front

Describe the stationary and mobile phase and the format for gas chromatography.

Back

Preview of the front of card 3

Card 4

Front

Describe the stationary and mobile phase and the format for liquid chromatography.

Back

Preview of the front of card 4

Card 5

Front

Describe the stationary and mobile phase and the format for supercritical chromatography.

Back

Preview of the front of card 5
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