Spin at fast speed. Heavier particles sediment the fastest.
1 of 5
Sucrose Density Centrifugation
Tube contains a sucrose gradient (more concentrated at the bottom). Samples added to the top and spun. Not an equilibrium method but better separation.
2 of 5
Isopycnic Centrifugation (Cesium Chloride Density Gradients)
Cesium Chloride reacts with water and creates a density gradient. Substances will pellet once they equal the density of the cesium solution.
3 of 5
Ultracentrifugation
Spin rotor at very high speeds. Can be analytical (study interactions or properties) or preparative (isolate and purify sample)
4 of 5
Microcentrifugation
Used for very small samples
5 of 5
Other cards in this set
Card 2
Front
Tube contains a sucrose gradient (more concentrated at the bottom). Samples added to the top and spun. Not an equilibrium method but better separation.
Back
Sucrose Density Centrifugation
Card 3
Front
Cesium Chloride reacts with water and creates a density gradient. Substances will pellet once they equal the density of the cesium solution.
Back
Card 4
Front
Spin rotor at very high speeds. Can be analytical (study interactions or properties) or preparative (isolate and purify sample)
Comments
No comments have yet been made