Cell Fractionation & Microscopes

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  • Created by: Voltace
  • Created on: 20-05-14 20:11
What are the three stages of cell fractionation?
Homogenisation , Filtration , Ultracentrigfugation
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What happens in homogenisation & filtration?
Homo - Cells grinded up, breaks plasma membrane, organelles released , isotonic & ice cold Filtration - passed through gauze, debris too large to fit through
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What happens in ultracentrigfugation?
Solution spun at high speed, pellet formed, new test tube supernatant spun again, Naughty Monkeys like Eating Rasins (Nuclei Mitochondria Lysosomes E.R Raisins)
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Positives & Negatives of Light Microscope
Positive: Living or Dead , Cheap Negative: Poor Resolution , Can't see Organelles
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Positives and negatives of Transmission & Scanning
Transmission: Positive- High Resolution Negative: Thin & Dead Scanning: 3D Image , Thick Specimen Negative: Non Living lower res than TEM
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Other cards in this set

Card 2

Front

What happens in homogenisation & filtration?

Back

Homo - Cells grinded up, breaks plasma membrane, organelles released , isotonic & ice cold Filtration - passed through gauze, debris too large to fit through

Card 3

Front

What happens in ultracentrigfugation?

Back

Preview of the front of card 3

Card 4

Front

Positives & Negatives of Light Microscope

Back

Preview of the front of card 4

Card 5

Front

Positives and negatives of Transmission & Scanning

Back

Preview of the front of card 5

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