Cell Structure and Microscopy 3.0 / 5 based on 1 rating ? BiologyCellular processes and structureA2/A-levelOCR Created by: 05friisbCreated on: 16-10-17 14:47 Why do microscope samples have to be thin? So light can shine through them. 1 of 30 What is dry mount? Samples are sectioned and placed under a cover slip. 2 of 30 What is wet mount? Samples are suspended in a liquid. 3 of 30 What is squash mount? Wet mount then another slide squishes the sample. 4 of 30 What is smear mount? Edge of a slide is used to smear the sample. 5 of 30 what does gram stain differentiate? gram + and gram - bacteria. 6 of 30 why does gram - bacteria lose its stain after being washed with alcohol? becuase it has a thinner cell wall. 7 of 30 how do you calculate magnification? magnification = size of image / actual size of object. 8 of 30 what are the two lenses in a light microscope? objective lens (near sample) and the eye piece lens. 9 of 30 what is magnification? how many times larger the image is than the actual size of the object being viewed. 10 of 30 what is resolution? The ability to distinguish two points separately. 11 of 30 millimeters to micrometers 1000 micrometers = 1 millimeter. 12 of 30 LSC microscope res and mag r: 0.1nm mag:100,000x 13 of 30 how lsc microscopes work? laser moves across a sample causing a fluorescent dye to emit light which is focused through a pinhole. 14 of 30 SE microscope res and mag r:0.1nm m:100,000x 15 of 30 how se microscopes work? electrons fired at a sample and they bounce off and are detected producing a 3D image 16 of 30 TE microscope res and mag r: 3-10nm m:500,000x 17 of 30 how te microscopes work? electrons fired at a sample and they are absorbed, denser parts absorb more electrons giving contrast in the black and white image. 18 of 30 what does the plasma membrane control? movement of substances in and out the cell. 19 of 30 what does the cell wall do? supports the plant cell 20 of 30 what does the nucleus do? controls the cells movement 21 of 30 what is the structure of the nucleus? nucleolus, chromatin, nuclear pores, nuclear evolope 22 of 30 what do lysosomes do? contain digestive enzymes and can be used to digest invading cells 23 of 30 what do the ribosomes do? synthesise proteins 24 of 30 what does the rough ER do? folds and process proteins 25 of 30 what does the smooth ER do? synthesises and processes lipids 26 of 30 what do vesicles do? transport substances in and out the cell 27 of 30 what does the golgi apparatus do? processes and packages proteins, they also produce lysosomes 28 of 30 what does the mitochondria do? aerobic respiration where ATP is produced 29 of 30 what is the purpose of chloroplast? where photosynthesis takes place 30 of 30
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