Cell Structure and Division - Eukaryotic Cells and Organelles

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How do you work out magnification?
Image/Actual
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What is a eukaryotic cell?
A cell with a nucleus and membrane bound organelles, with no cell wall.
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Why might the shape of ONE organelle may appear to be different?
It can be seen transverse or longitudinal
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What is the folded membrane of a mitochondrion called?
cristae
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What in a chloroplast contains chlorophyll?
grana
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What is the process called that secretes protein?
exocytosis
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What are prokaryotes?
single celled organisms
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What modifies proteins?
Golgi apparatus
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Name one difference between a prokaryotic and eukaryotic cell
prokaryotic cells have a loop of DNA
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Why is it best to use an optical microscope?
Results in a colored image
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why is it better to use an SEM microscope rather then a TEM microscope?
It isn't as complex so doesn't take as long to prep
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How do viruses reproduce?
Inject their DNA into host cells. This cell then divides.
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which part of the virus is made of fat?
Lipid envelope
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What is a protein coat called (virus)?
capsid
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What does resolution mean?
How detailed an image is.
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Cell fractionation by...
ultracentrifugation
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What is the aim of cell fractionation?
To obtain undamaged cells of one type.
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What is the first step of CF?
The cell is cut into small pieces to increase surface area
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What are the 3 conditions that the cell is placed in to during the second stage of CF?
cold, isotonic, buffer
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Why are the conditions cold for CF?
Less kinetic energy, so enzymes don't function.
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Why do the conditions for CF have to be isotonic?
Prevents osmosis, so there is no damage to cells.
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Why does buffer have to be added during CF?
It is a chemical that resists pH change. Enzymes do NOT denature.
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What is the 3rd stage of CF?
Homogenised and filtered.
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What are the cells spun in during CF? And what are the conditions for the first time?
A centrifuge. Short time, low speed.
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What is the liquid substance after CF? What are the dense organelles called?
Supernatant. Pellet.
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What is the role of the nucleoli?
Make ribosomes
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Other cards in this set

Card 2

Front

What is a eukaryotic cell?

Back

A cell with a nucleus and membrane bound organelles, with no cell wall.

Card 3

Front

Why might the shape of ONE organelle may appear to be different?

Back

Preview of the front of card 3

Card 4

Front

What is the folded membrane of a mitochondrion called?

Back

Preview of the front of card 4

Card 5

Front

What in a chloroplast contains chlorophyll?

Back

Preview of the front of card 5
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