BMS1031 Bacterial Genetics Example Q's

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1. Repressor proteins:

  • B. bind to RNA polymerase
  • C. can bind to more than one DNA sequence at the same time
  • A and C
  • A. negatively regulate gene expression
  • D. act positively on gene expression
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2. cDNA cloning:

  • requires the enzyme reverse transcriptase
  • does not require thought
  • requires genomic DNA
  • does not require ligation
  • requires bacteriophages

3. The ligand that binds to the E.coli CRP (CAP) protein is:

  • cyclic AMP
  • cycteine
  • tryptophan
  • glucose
  • arabinose

4. Mutation of an adenine bas to guanine base in DNA is called...

  • a silent mutation
  • a transversion mutation
  • a transition mutation
  • a frameshift mutation

5. Which amino acid does the tRNA 5'-AAC-3' code?

  • Valine (GUU)
  • Lysine (UUA, CUU etc)
  • Asparagine (GAU)
  • Glutamine (GAA)
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