BMS1031 Bacterial Genetics Example Q's

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1. transposons:

  • all of the above
  • encode a transposase enzyme
  • can generally insert into any DNA sequence
  • have inverted repeats at each end
  • generate a small tandem sequence duplication when they transpose
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2. which codon serves as the start codon in mRNA for translation?

  • AUG
  • UGG
  • AGU
  • UGA

3. Mutations in lacO, the operator of the lac operon prevent:

  • binding of Lacl repressor
  • none of the above
  • binding of RNA polymerase
  • expression of the lac operon
  • binding of CRP

4. Genetic engineering generally requires the following resources?

  • a transformation system
  • all of the above
  • cloning vectors
  • restriction enzymes
  • DNA ligase

5. To express a eukaryotic gene in a bacterium:

  • C. the genomic DNA first needs to be cloned from the eukaryotic genome
  • A. a bacterial expression vector must be used
  • A and B
  • B. firstly cDNA has to be synthesised from the mature mRNA before cloning the gene
  • all of the above


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