BMS1031 Bacterial Genetics Example Q's

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1. The formation of the 2:3 stem-loop in the trpL leader RNA of the E.coli trp operon:

  • prevents the formation of the 3:4 stem loop
  • all of the above
  • is facilitated when Trp levels in the cell are low
  • prevents attenuation of the trp operon
  • occurs when the ribosome stalls in trpL
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2. which codon serves as the start codon in mRNA for translation?

  • AUG
  • AGU
  • UGA
  • UGG

3. cDNA cloning:

  • does not require thought
  • requires genomic DNA
  • requires the enzyme reverse transcriptase
  • does not require ligation
  • requires bacteriophages

4. In the presence of L-arabinose and absence of glucose which transcription factors are bound to the upstream promoter region of E.coli araBAD operon?

  • CRP protein
  • Lacl repressor and AraC
  • CRP and AraC
  • AraC protein
  • lacl repressor

5. How many different transcription factors are bound to the E.coli lac operon promoter region when both lactose and glucose are present in the growth medium?

  • 4
  • 1
  • 2
  • none
  • 3

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