BMS1031 Bacterial Genetics Example Q's

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1. cDNA cloning:

  • requires bacteriophages
  • does not require thought
  • requires the enzyme reverse transcriptase
  • does not require ligation
  • requires genomic DNA
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2. Mutations in the Lacl gene, encoding the Lacl repressor proteins:

  • generally lead to constitutive expression of the lac operon
  • all of the above
  • prevent binding of RNA polymerase
  • are cis-dominant
  • prevent growth of lactose

3. which codon serves as the start codon in mRNA for translation?

  • UGG
  • AUG
  • UGA
  • AGU

4. Horizontal (lateral) gene transfer in bacteria can occur via:

  • transcription
  • replication
  • recombination
  • cojugation
  • transposition

5. Which one of the following codons could be mutated to a stop codon by single base substitution (point mutation)?

  • UUA
  • AUC
  • UUC
  • UUU
  • ACU

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