BMS1031 Bacterial Genetics Example Q's

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1. To express a eukaryotic gene in a bacterium:

  • B. firstly cDNA has to be synthesised from the mature mRNA before cloning the gene
  • A and B
  • all of the above
  • A. a bacterial expression vector must be used
  • C. the genomic DNA first needs to be cloned from the eukaryotic genome
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2. The trp operon attenuation mechanism..

  • relies on the coupling of transcription and translation
  • requires the binding of the TrpR repressor
  • relies on the coupling of replication and translation
  • relies on the coupling of replication and translation

3. Which amino acid does the tRNA 5'-AAC-3' code?

  • Valine (GUU)
  • Glutamine (GAA)
  • Lysine (UUA, CUU etc)
  • Asparagine (GAU)

4. When E.coli is grown on a mixture of lactose and glucose?

  • lactose represses glycolysis
  • lactose and glucose are used at the same time
  • lactose is used first
  • glucose is used first
  • lactose is never used

5. Lambda bacteriophage:

  • can enter either a lytic or lysogenic growth phase
  • never lyses the host cell
  • always integrates into the hosts chromosome
  • is transfered via conjugation
  • always lyses the host cell

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