biology - enzyme experiments

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how can you prevent the enzymes dissolving in the mixture?
immobalising them in alginate beads
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what are immobalised enzymes used for?
making lactose free milk
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how do you make the alginate beads?
1.) mix the sodium alginate with lactase in a syringe, 2.) add mixture to a beaker of calcium chloride one drop at a time, 3.) leave the beads to harden.
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what do you do once the alginate beads are made?
put some nylon gauze in a syringe and attach a tap to the end, then add the beads
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how do you make the lactose free milk?
1.) test the untreated milk with a glucose strip 2.) add the milk to the syringe, collect the treated milk in a small beaker 3.) test the content of the treated milk with a glucose strip, and compare the strips together.
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how is the juice of fruit extracted?
by the use of pectinase
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how do you extract the juice out of apples?
1.) chop an apple into small chunks and divide into 2 beakers, 2.) add pectinase solution to one of the beakers and add the same volume of water to the other, 3.) incubate in a water bath 40 degrees for 15 mins, 4.) filter 5.) record the juice vol
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Card 2

Front

what are immobalised enzymes used for?

Back

making lactose free milk

Card 3

Front

how do you make the alginate beads?

Back

Preview of the front of card 3

Card 4

Front

what do you do once the alginate beads are made?

Back

Preview of the front of card 4

Card 5

Front

how do you make the lactose free milk?

Back

Preview of the front of card 5
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