Biology Biotechnology Definitions

HideShow resource information

1. Batch culture is where

  • 1) enzymes not present with products so downstream processing cost low 2) enzymes are immediately available for reuse 3) immobilised enzymes are more stable as the matrix protects them
  • A fixed quantity of nutrient is added and products are removed at the end of the period, useful for secondary metabolite production
  • 1) immobilisation requires additional time, equipment and materials 2) immobilised enzymes may be less active as they are held separate 3) any contamination is costly as the whole system would need to be stopped
1 of 20

Other questions in this quiz

2. The standard growth curve for a culture of microorganisms is labelled

  • Lag, Log, Stationary, Death
  • 1) enzymes not present with products so downstream processing cost low 2) enzymes are immediately available for reuse 3) immobilised enzymes are more stable as the matrix protects them
  • 1) immobilisation requires additional time, equipment and materials 2) immobilised enzymes may be less active as they are held separate 3) any contamination is costly as the whole system would need to be stopped
  • A growth of microorganisms, this may be a single species (pure culture) or a mixed species (mixed culture), microorganisms can be cultured in a liquid such as nutrient broth or a solid surface such as nutrient agar gel

3. The disadvantages of immobilising enzymes are

  • 1) immobilisation requires additional time, equipment and materials 2) immobilised enzymes may be less active as they are held separate 3) any contamination is costly as the whole system would need to be stopped
  • A growth of microorganisms, this may be a single species (pure culture) or a mixed species (mixed culture), microorganisms can be cultured in a liquid such as nutrient broth or a solid surface such as nutrient agar gel

4. The two main methods of artificial vegetative propogation are

  • Large scale cloning
  • 1) Taking cuttings - a section of the stem is cut between leaf joints, treated with hormones and then planted 2) grafting - a shoot section is joined to an already growing root and stem, the graft is identical to the parent but not the rootstock
  • Ethical objections, and some people think there is a lack of understanding on how these clones will behave over time

5. Outlined steps of micropropogation: tissue culture

  • 1) explant taken 2) sterilised 3) placed on nutrient growth medium 4) cells divide but do not differentiate forming callus 5) single callus cells placed on shoot growth hormones 6) then placed on root growing hormones 7) acclimatised (greenhouse)
  • 1) Taking cuttings - a section of the stem is cut between leaf joints, treated with hormones and then planted 2) grafting - a shoot section is joined to an already growing root and stem, the graft is identical to the parent but not the rootstock
  • Refers to the separation of cells of any tissue type and their growth in or on a nutrient medium containing plant hormones that stimulate development of the complete plant

Comments

No comments have yet been made

Similar Biology resources:

See all Biology resources »See all Practical applications of biology resources »