Biology - Microscopes

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What microscope is commonly used in school?
Optical
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Where do we place the microscope slide?
On the stage.
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The stage has ___ to hold the slide down.
Clips
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The lamp is below the ___
Stage
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What does the lamp do?
Shines light so it passes through the slide
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Some microscopes have a mirror instead of a lamp. The mirror can be used to reflect the light instead. What type of microscope has a mirror?
Optical. Some optical have lamps, some have mirrors.
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What's above the stage?
Objective lenses
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How many are there?
3
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What are the three magnifications?
4x 10x 40x
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Some microscopes, such as the optical one, have limited resolution. What is meant by this?
Image is blurred
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At the top of the microscope is the ___ (where you look down)
eyepiece
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The eyepiece lens has a mag. of...
10x
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There are two dials on a optical microscope. One is large and short, and one is thin and tall. Which one's which?
Coarse focusing dial is the larger one. Fine focusing dial is the thinner one.
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What does the coarse dial do?
Lowers/highers the objective lens.
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What does the fine dial do?
Focuses the lens clearly
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To view a prepared slid with the microscope, what do you need to do first?
Place slid on stage, and use clips to secure it.
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Then which lens should you use the view the slid first?
Lowest power one (4x)
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How do you position the lens?
Just above slide by using the coarse focusing dial, watching from the slide for accuracy
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Why shouldn't you look down the eyepiece when lowering lens?
Risks damaging the slid
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We now look down the eye piece and higher the lens until it comes into focus. We then use the fine focusing dial to...
Bring it into clear focus
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How do you calculate magnification from knowing an image size and the real size?
image size / real size = mag
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How do you calculate total mag. from the lenses mag.?
Times the eyepiece with objective lenses mag (e.g 10x times 4x)
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What's the advantage of using an electron microscope instead of an optical?
Electron has better mag. and reolution
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What would you be able to see in an anime cell with an optical microscope?
Cell membrane, cytoplasm, nucleus and maybe mitochondria
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What about in a plant cell?
Cell wall, cytoplasm, nucleus and maybe vacuole and chloroplast
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An image size is 45mm. The actual size is 0.01mm. Calculate mag.
45/0.01 = 4500X
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Other cards in this set

Card 2

Front

Where do we place the microscope slide?

Back

On the stage.

Card 3

Front

The stage has ___ to hold the slide down.

Back

Preview of the front of card 3

Card 4

Front

The lamp is below the ___

Back

Preview of the front of card 4

Card 5

Front

What does the lamp do?

Back

Preview of the front of card 5
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