BIOL114 - Lecture 3 - Gene Expression analysis

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  • Created by: Katherine
  • Created on: 15-03-16 14:33
How can we control gene expression?
Either by controlling transcription (how easy it is for a gene to be transcribed into mRNA) or you can control translation and how the mRNA is converted into a protein.
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Is mRNA stable?
No, it is inherently unstable.
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What might you want to know about gene expression?
The level of gene expression, this might be relative (you might want to know if a gene is more expressived in the gene or stem) or absolute (is the gene on or off). Or you might want to have a temporal look at when expression takes place.
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What are the techniques used for measuring mRNA levels?
Northen blotting, RT-PCR, Microarray .
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Techniques for measuring protein levels:
Western blotting.
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What is Northern blotting?
Is is the opposite of southern blotting and it looks at RNA.
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What is Reverse Transcriptase PCR? (RT-PCR)/
It is simple,quick and cheap - replaced northern blotting for routine analysis.
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What is the process of RT-PCR?
You start with DNA in the nucleus and mRNA in the cytoplasm. Extract the mRNA and you put in a primer and you make the first strand of DNA. You break down RNA, and make a second DNA strand.
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Which enzyme breaks down the RNA strand?
RNAse H - got to 26:14 minutes
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Card 2

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Is mRNA stable?

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No, it is inherently unstable.

Card 3

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What might you want to know about gene expression?

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Card 4

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What are the techniques used for measuring mRNA levels?

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Card 5

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Techniques for measuring protein levels:

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