Cells are homogenised to break them open; cell debris and whole cells are filtered off, leaving a suspension
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What happens during ultracentrifugation?
The suspension is centrifuged, at a low speed; most dense organelles (nucleus) separates to bottom. (Leaves supernatant); supernatant spun at higher speed etc.
What are the 3 conditions that are needed for this process?
Cold temperature, Isotonic, Buffer
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What does it have to be at a cold temperature?
Slow down enzyme activity, so prevent digestion of organelles
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Why is there a buffer solution?
To control the pH level, to prevent denaturation of the proteins (and enzymes)
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Why is it isotonic?
Prevents osmotic lysis or the organelles (bc. same water potential)
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What does isotonic mean, in this case?
The solution outside the organelles and inside the organelles have the same water potential - the same concentration of water
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Other cards in this set
Card 2
Front
What happens during ultracentrifugation?
Back
The suspension is centrifuged, at a low speed; most dense organelles (nucleus) separates to bottom. (Leaves supernatant); supernatant spun at higher speed etc.
Card 3
Front
What is the order of density of organelles?
Back
Card 4
Front
What is centrifugation/a centrifuge?
Back
Card 5
Front
What are the 3 conditions that are needed for this process?
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