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  • Created by: GGB2016
  • Created on: 21-06-16 17:28

1. Why is the DNA sample cooled from 70 degrees to 55 degrees?

  • Allows primers to attach to DNA strands and mark where DNA polymerase attaches
  • To allow reverse transcriptase to convert RNA to DNA
  • Allows the hydrogen bonds between the two strands of the DNA to break causing them to separate
  • Allows DNA polymerase to add free nucleotides to make a new molecule of DNA
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2. What is eugenics?

  • Encouraging desirable traits and selective breeding of humans
  • Encouraging people to use pre natal and post natal screening
  • Encouraging gene therapy
  • Encouraging the use of stem cells instead of embryo cloning

3. Which of these is NOT used to form recombinant DNA?

  • Reverse transcriptase
  • DNA ligase
  • RNA polymerase
  • DNA polymerase
  • Restriction endonucleases

4. What are stem cells?

  • Cells that produce cancer
  • Cells that are undifferentiated but can become specialised
  • Cells that are not totipotent and can only produce 1 cell type
  • Cells that have become differentiated

5. What is recombinant DNA?

  • Foreign DNA inserted into another section of DNA
  • Undifferentiated cells that have the potential to become specialised
  • DNA that codes for a non functional protein
  • Incorrect DNA


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