3B Lab Test 2k18

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Which lymph nodes are located under the arms?
Brachial lymph nodes
1 of 23
Which organ sits just above the heart?
Thymus
2 of 23
Which marker is used to label astrocytes?
Glial fibrillary acidic protein
3 of 23
In immunocytochemistry, which species should the blocking buffer derive from?
The same species as the secondary antibody
4 of 23
Which cells produce myelin sheaths?
Oligodendrocytes
5 of 23
Which substance forms the apoptosome?
Cytochrome c
6 of 23
Which event in apoptosis is measured using JC-1 staining?
Mitochondrial depolarisation
7 of 23
Which event in apoptosis is measured using Annexin-V?
Phosphatidyl-serine translocation
8 of 23
What is the role of caspase 1 in pyroptosis?
Creates pores in cell membrane for cytokine release
9 of 23
Which method is not used to measure pyroptosis?
TUNEL assay
10 of 23
Lactate dehydrogenase is a marker for which process?
Non-specific cell lysis
11 of 23
Which lymph nodes are found within the intestines?
Mesenteric lymph nodes
12 of 23
What is the purpose of doublet discrimination gating?
Eliminates aggregated cells
13 of 23
Which parameter is the threshold usually set on in flow cytometry?
FSC-A
14 of 23
What does forward scatter in flow cytometry show?
Cell size
15 of 23
What chart is used to show results from a single channel in flow cytometry?
Histogram
16 of 23
Which plot is usually displayed with a logarithmic scale?
Contour plot
17 of 23
Which index is used to determine flurophore brightness?
Staining index
18 of 23
Why is compensation necessary in flow cytometry?
To reduce results with overlapping fluorescence
19 of 23
What are compensation beads?
Small synthetic particles coated with antibodies specific for light chains
20 of 23
What are isotype controls used to determine?
Levels of non-specific binding
21 of 23
Which is the best way to determine positive cells in an experiment?
Fluorescence minus one
22 of 23
What is doublet discrimination associated with?
Poor sample preparation
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Other cards in this set

Card 2

Front

Which organ sits just above the heart?

Back

Thymus

Card 3

Front

Which marker is used to label astrocytes?

Back

Preview of the front of card 3

Card 4

Front

In immunocytochemistry, which species should the blocking buffer derive from?

Back

Preview of the front of card 4

Card 5

Front

Which cells produce myelin sheaths?

Back

Preview of the front of card 5
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