Required Practical 2: Culturing Microorganisms


Required Practical 2: Culturing Microorganisms 

I should be able to: 

  • 1. Describe how to prepare an uncontaminated bacterial culture using aseptic technique. 

  • 2. Describe how to investigate the effect of antibiotics on bacterial growth. 




  • First, sterilise all Petri dishes, bacterial nutrient broth and agar. 

  • This kills any unwanted microorganisms and it prevents contamination.  

  • Bacteria are normally transferred into the culture using an inoculating loop.  

  • We sterilise the inoculating loop by passing it through a Bunsen burner flame.  

  • Next, we attach the lid of the Petri dish using


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