Required Practical 2: Culturing Microorganisms

Required Practical 2: Culturing Microorganisms 

I should be able to: 

• 1. Describe how to prepare an uncontaminated bacterial culture using aseptic technique. 

• 2. Describe how to investigate the effect of antibiotics on bacterial growth. 

1. 

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• First, sterilise all Petri dishes, bacterial nutrient broth and agar. 

• This kills any unwanted microorganisms and it prevents contamination.  

• Bacteria are normally transferred into the culture using an inoculating loop.  

• We sterilise the inoculating loop by passing it through a Bunsen burner flame.  

• Next, we attach the lid of the Petri dish using…