PROCESSES I MUST LEARN

Processes I must learn!!!!

Cell fractionation:

• The tissue from which the organelles are to be harvested from is firstly placed in a COLD, ISOTONIC, BUFFERED solution

  The solution is cold to minimise enzyme activity

  The solution is isotonic to prevent organelle damage due to osmotic water gain or loss

  The solution is buffered to maintain a constant pH

   

• The solution containing the cells is then HOMOGENISED in a blender to release organelles from the cells. After homogenising, the fluid is known as the HOMOGENATE, it is now FILTERED to remove any large pieces of cell organelles

   

• After removing the large pieces of cells then the they are spun…