Population growth in a closed system
- Created by: mutsa__
- Created on: 28-02-17 20:29
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Serial dilutions:
- In order to investigate rate of growth of population of microorganisms it is essential to reduce population density
- This can be achieved using serial dilution
- This is a step-wise broth dilution:
- At each step the broth is diluted by 10cm^3 until a series of dilutions are formed with suitable labels
- Each dilution is used to inoculate an agar plate and one will produce a culture plate in number of colonies can be counted
- Population density: dilution factor x 10 x volume added to plate
The growth curve:
- Small population of microorganisms in a closed culture that contains all nutrients required for growth will undergo population growth
LAG phase:
- In the early stages of population growth as population is still small and organisms are adjusting to their new environment
- …
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