Population growth in a closed system

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  • Created by: mutsa__
  • Created on: 28-02-17 20:29

Serial dilutions:

  • In order to investigate rate of growth of population of microorganisms it is essential to reduce population density 
  • This can be achieved using serial dilution 
  • This is  a step-wise broth dilution:
    • At each step the broth is diluted by 10cm^3 until a series of dilutions are formed with suitable labels 
  • Each dilution is used to inoculate an agar plate and one will produce a culture plate in number of colonies can be counted 
  • Population density: dilution factor  x 10 x volume added to plate 

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The growth curve:

  • Small population of microorganisms in a closed culture that contains all nutrients required for growth will undergo population growth 

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LAG phase:

  • In the early stages of population growth as population is still small and organisms are adjusting to their new environment 

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