model organsims
- Created by: claudsia
- Created on: 07-01-22 14:43
Model Plants
Arabidopsis - small genome
- 4-6 week life cycle
- easy to genetically modify
Modifying a plant genetically
- Agrobavterium is a pathogen that causes tumour formation for its propagation. This taken with genome insertion function via TI plasmid was rendered non pathogenic. Can hop genomes - TDNA randomly inserts in plant DNA without killing - inserting genes of interest to transform the plant or knock out genes
*Agrobacterium transformation: A gene of 'interest' is inserted into the T-DNA of the TI plasmid of the agrobacterium.
- easy to do as you can just dip the plant in agrobacterium culture and the rest occurs aturally.
Rice Oryza
Good model plant because:
- we eat it
- rapid life cycle - not as fast as AB
- good seed set - lots p/ plant in good conditions
- can transform w agrobacterium
POPLAR populus
- a tree
- slow groing , lifespan
perennial not annual like AB
- Age related changes in development e.g. change to flowering stages happens at v specific times
- - Relatively short life cycle can prod little trees in 3 -4 years. Flower 3-6 but can be sped by gene modification so can flower in year
- can b etransformed by agro an dother methods
- dioecious = sep male and fem parts - longer propagation time tho
MOSS PHYSCOMITRELLA PATENS
- small sequence genome
- short life cycle (months)
- easy to grown in lab
- homologous recombination - targeted gene replacement
- mainly haploid during llife cycle
angiosperms = flowering plants
Are model organism still relevant
benefits of using non model plants:
- specific biology that *** be studied in the generic model
- bigger cells means for…
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