model organsims

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  • Created by: claudsia
  • Created on: 07-01-22 14:43

Model Plants

Arabidopsis - small genome

- 4-6 week life cycle

- easy to genetically modify

Modifying a plant genetically

- Agrobavterium is a pathogen that causes tumour formation for its propagation. This taken with genome insertion function via TI plasmid was rendered non pathogenic. Can hop genomes - TDNA randomly inserts in plant DNA without killing - inserting genes of interest to transform the plant or knock out genes

*Agrobacterium transformation: A gene of 'interest' is inserted into the T-DNA of the TI plasmid of the agrobacterium.

- easy to do as you can just dip the plant in agrobacterium culture and the rest occurs aturally.

Rice Oryza

Good model plant because:

we eat it

- rapid life cycle - not as fast as AB

- good seed set - lots p/ plant in good conditions

- can transform w agrobacterium

POPLAR populus

- a tree

- slow groing , lifespan

perennial not annual like AB

- Age related changes in development e.g. change to flowering stages happens at v specific times

-          Relatively short life cycle  can prod little trees in 3 -4 years. Flower 3-6 but can be sped by gene modification so can flower in year

- can b etransformed by agro an dother methods

- dioecious = sep male and fem parts - longer propagation time tho

MOSS PHYSCOMITRELLA PATENS

- small sequence genome

- short life cycle (months)

- easy to grown in lab 

- homologous recombination - targeted gene replacement

- mainly haploid during llife cycle

angiosperms = flowering plants

Are model organism still relevant

benefits of using non model plants:

- specific biology that *** be studied in the generic model

- bigger cells means for…

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