Investigating enzymatic reactions


The enzyme amylase catalyzes the breakdown of starch to maltose. It is easy to detect starch using iodine solution:

  • if starch is present, the iodine solution will change from browny-orange to blue-black


1. put a drop of iodine solution into every well of a spotting tile

2. set up the bunsen burner on a heat-proof mat with a tripod and gauze over it, place a beaker of water on top and heat the water till it's 35C- use a thermometer to measure temperature

3. use a syringe


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