In Vitro and In Vivo Cloning

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  • Created by: Abi
  • Created on: 06-06-11 11:59

In Vitro - Polymerase Chain Reaction (PCR)

Needed:

Heat Cycler

Free Nucleotides

DNA Polymerase (Heat Stable, from Thermus Aquaticus bacteria)

Piece of DNA

Primers for start and end of gene

Stages:

1. 95C - Separates two stranges of DNA; Breaks hydrogen bonds in helix.

2. 55C- Primer attaches to open strands to stop strands from joining back together.

3. 72C- DNA polymerase joins the free nucleotides to make the…

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