In Vitro - Polymerase Chain Reaction (PCR)
DNA Polymerase (Heat Stable, from Thermus Aquaticus bacteria)
Piece of DNA
Primers for start and end of gene
1. 95C - Separates two stranges of DNA; Breaks hydrogen bonds in helix.
2. 55C- Primer attaches to open strands to stop strands from joining back together.
3. 72C- DNA polymerase joins the free nucleotides to make the…