genetic engineering - by recominant DNA
- Created by: rebecca_edwards
- Created on: 19-03-16 09:09
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Recombinant DNA
Topics:
- define the term recombinant DNA;
- explain that genetic engineering involves the extraction of genes from one organism, in order to place them in another organism
- describe how sections of DNA containing a desired gene can be extracted from a donor organism using restriction enzymes;
- explain how isolated DNA fragments can be placed in plasmids, with reference to the role of ligase;
- state other vectors into which fragments of DNA may be incorporated;
- explain how plasmids may be taken up by bacterial cells in order to produce a transgenic microorganism that can express a desired gene product;
- outline how genetic markers in plasmids can be used to identify the bacteria that have taken up a recombinant plasmid;
Important Points:
recombinant DNA; A section of DNA, often in the form of a plasmid, which is formed by joining DNA sections from two different sources- DNA ligase joins together the phosphate sugar backbones from the plasmid and the desired gene, to form recombinant DNA
- Density gradient ultacentrifugation and electrophoresis are also carried out!
Lecture Topic:
Process: genetic engineering by recombinant DNA
- Once a desired gene has been identified, it can be cut from DNA using a restriction enzyme
- A plasmid is a small circular piece of DNA. Plasmids often carry genes that code for resistance to antibiotic chemicals.
- plasmids are cut with the same restriction enzyme as that used to isolate the gene, then complementary stickyends will be formed
- The restriction enzyme cuts at the palindromic parts
- Howto cut:
- Add a set of DNA
- To buffer solution
- To restriction enzymes
- Tube is closed
- Contents mixed
- Transfers to 72.c water bath
- Add ED TA to stop enzyme action
- Mixing quantities of plasmid and gene in the presence of ligase enzyme allows some plasmids to combine with the gene,
- which then becomes…
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