Effects on the Rate of Enzyme Activity

Temperature:

• Increasing Temperature -
  • increases kinetic energy
  • increases number of collisions between enzymes and substrates as there is more movement
  • enzyme - substrate form more often
  • rate of reaction increases
• Above Optimum Temperature - 
  • enzyme's active site begins to denature 
  • hydrogen bonds break causing the tertiary structure to change
  • substrates can't bind
  • enzyme - substrate complexes can't form
  • rate of reaction decreases

pH:

• At Optimum pH - 
  • enzyme - substrate complexes can form
  • active site is complementary
  • substrate can bind
• …