Cell Fractionation

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If you want to look at organelles under an electron microscope first you have to separate them from the rest of the cell. There are three steps to this process;

1. Homogenisation

  • This breaks up the plasma membrane and releases the organelles into  a solution.
  • Homogenisation can be done in several different wasy e.g. vibration the cells, grinding them up in a blender.

2. Filtration

  • The homogenised sloution  is filtered through a gauze to separate any large


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