BY5.6 Genetic Fingerprinting
- Created by: Yazmin Ali
- Created on: 21-06-16 20:46
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Genetic Fingerprinting
- 90% DNA on human chromosome has no function
- individuals acquire different sequences of non fucntional DNA
- vary in length but consist of sequences of bases, 20-40 bases long, often repeated many times
- unique lenghts or non coding DNA are called hyper varibale regions (HVR) or short tandem repears (STRs)
- number og time the lenghts are repeated is used to show the difference between individuals
- DNA is extracted from the sample and cut into small fragments using restriction endonculeases
- DNA is separated using electrophoresis
- the smaller the fragmen, the farther it moves and DNA becomes eparated into bands according to fragment size
- trough containing the gel is covered with a nylon membrane and the fragments are transferred to it by a process called Southern Blotting
- nylon membrane…
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