Automated DNA sequencing.
This method of DNA sequencing involves a mixture of DNA polymerase, many copies of a single-stranded template DNA fragment, free nucleotides and primers. Within the free nucleotides, however, are modified nucleotides that carry a fluorescent marker.
It begins as in PCR.
1) the primer joins at the 3' end of the template strand, allowing DNA polymerase to attach.
2) DNA polymerase attaches the free nucleotides according…