- Created by: Billie2503
- Created on: 30-11-19 10:41
- used to separate stuff in a mixture
- once separated you can identify the components
- it can separate out and identify biological molecules e.g. amino acids, carbohydrates, vitamins and nucleic acids
- there are different types- thin layer and paper chromotography
How does it work?
- a mobile phase- where the molecules can move, this is a liquid solvent, such as ethanol or water
- a stationery phase- where the molecules can't move. in paper chromatography this is a piece of chromatography paper. in thin layer this is a thin layer of solid (0.1-0.3mm thick) e.g. silica gel, on a glass plate.
- they all use the same basic principle:
- the mobile phase moves through/ on the stationary phase
- the components spend different amounts of time in the mobile and stationery phase
- the components that spend longer…