(4) Gene Transfer

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Technique for gene transfer

Gene transfer involves the following elements: 

- vector to carry the gene into the host cell. (plasmids are very common) 

- host cell

- restriction enzymes

- DNA ligase

Bacteria carry al required genetic info on one large circular DNA. They also possess extra DNA in the form of plasmids. 

(http://2.bp.blogspot.com/-bpILr3UYEVY/UEWSXwbjZCI/AAAAAAAAJXM/eGpqsvBlj9s/s640/GM+plasmid.gif)

1. spice the desired gene into a plasmid and transfer it into a bacterial cell. 

2. Culture bacteria, many will have have a plasmid with the desire gene. 

3. Use a section of nucelotides completementary to the desired genes but also…

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