Technique for gene transfer
Gene transfer involves the following elements:
- vector to carry the gene into the host cell. (plasmids are very common)
- host cell
- restriction enzymes
- DNA ligase
Bacteria carry al required genetic info on one large circular DNA. They also possess extra DNA in the form of plasmids.
1. spice the desired gene into a plasmid and transfer it into a bacterial cell.
2. Culture bacteria, many will have have a plasmid with the desire gene.
3. Use a section of nucelotides completementary to the desired genes but also…