- George and Lennie - two migrant workers.
- Carlson and Whit - workers on the ranch.
- Crooks - A stable hand.
- Curley and Curleys wife - The boss's son and his new wife.
- The boss- The man in charge of the ranch
- Slim- Head of one of the grain teams.
- Candy and his dog - An old man who cleans the ranch and his dog.
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