- Make up a range of caffeine solutions of different concentrations and a control.
- Transfer one Daphnia into the dimple on a cavity slide (a microscope slide with a rounded dip).
- Place the slide onto a light microscope and focus it on the beating heart of the Daphnia.
- Place a small drop of caffeine solution onto the slide.
- Count the number of heartbeats in 10 seconds and multiply this by 6 to get beats per minute.
- Repeat this 10 times using the same concentration but a different Daphnia each time.
- Repeat the experiment using all the caffeine solutions and the control.
- Draw a graph to compare the results.
It is unethical to cause distress to any of the Daphnia, you can reduce stress by making sure the light on the microscope isn’t left on too long so it doesn’t make them too hot.
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