DNA sample is mixed with a supply of DNA nucleotides and DNA polymerase
Mixture is heated to 95°C which breaks hydrogen bonds holding the complementary bases, the sample becomes single stranded.
Primers or Short lengths of single-stranded DNA are added (10-20 bases)
The temperature is reduced to 55°C, allowing primers to bind and form small double stranded sections of DNA at either end.
DNA Polymerase can bind to these double-stranded sections
DNA sample is mixed with a supply of DNA nucleotides and DNA polymerase
DNA polymerase is described as thermophilic, it isn't denatured at hight temperatures used in the process. It is derive from a thermophilic bacterium, Thermus aquaticus.
Mixture is heated to 95°C which breaks hydrogen bonds holding the complementary bases, the sample becomes single stranded.
Primers or Short lengths of single-stranded DNA are added (10-20 bases)
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