Ecological barriers - There is a physical barrier seperating two groups of organisms, resulting in them evolving into different species. eg. mountain ranges.
Temporal barriers - Unable to interbreed due to being reproductively active at different times of the year.
Reproductive barriers - There are reasons why they cannot reproduce:
1. They have different courtship behaviour.
2. Mechanical problems with mating eg. size differences.
3. Gamete incompatibility - Gametes will not fuse to produce an zygote.
4. Zygote inviability - The zygote is unable to develop properly, so it dies.
5. Hybrid sterility - Any offspring are unable to produce gametes.
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