-Solvent is forced through the column at high pressure of up to 400 atmosphers
-It allows you to use smaller particle size for column packing material giving it greater SA for interactions between stationary phasr and the molecules flowing past it.
*Normal Phase:
-polar stationary phase (silica) and non polar mobile phase (hexane)
-molecules elute in order of increasing polarity
-The more polar the mobile phase, the faster the elution of organic compound
*Reverse Phase:
-Non polar stationary phase (octadecysilane coated (ODS) silical gel) and polar mobile phase (water, methanol)
-The more lipophilic the mobile phase, the faster the elution of organic compound
Elution of ionisable components: alter pH of mobile phase
Detectors:
-UV absorption or Fluorescence detector
-Refractive index detector
Comments
No comments have yet been made