OCR Biology F215 - Biotechnology and gene technology exam questions tricky questions from exam papers 3.0 / 5 based on 3 ratings ? BiologyPractical applications of biologyA2/A-level Created by: sophieCreated on: 11-04-12 15:31 Penicillin Production Explain the fall in Lactose and ammonia when producing penicillin Both used for growth and to make penicillin Lactose broken down to glucose for respiration Ammonia used to make protein/amino acids/nucleotides/nucleic acids 1 of 9 Explain the importance of maintaining aseptic cond Avoid unwanted microbe presence no competition for nutrients conditions remain unchanged no contamination of penicillin batch prevent escape of microbes/fungus/spores Give the name of the large pressure cooker used to steam-sterilise equipment autoclave 2 of 9 Name each of the following descriptions (gene tech An enzyme that synthesises new DNA DNA polymerase An enzyme that cuts DNA at specific sequenceses Rrestriction enzyme An enzyme that reseals the cut ends of DNA DNA ligase Small cercular pieces of DNA found in bacteria containing antibiotic resistance genes Pasmids An enzyme found on some viruses with an RNA genome: enzyme that converts RNA to DNA Reverse transcriptase 3 of 9 Explain the advantages of plant growth by tissue c All genetically identicle All disease free Can be genetically modified Easier - all ready to pick at same time, bulk production Rapid ripening Uniform/desired characteristic 4 of 9 Suggest consequences of widespread cultivation of All genetically uniform, no variety, narrow gene pool Decreased chance of mutation No variation to adventatious characteristics All susceptible to same disease, all wiped out 5 of 9 Describe how a prokaryote divides Binary fission DNA replicates Cell wall grows (elongates) cell splits by cytokinesis daughter cell is genetically identical to parent cell 6 of 9 Explain how to change a lab batch fermenter into a Reservoir for nutrient storage Steady input of nutrients steady removal of products and toxins at same rate as input keep same volume maintain pH, O2 and temp levels using sensors/probes Maintain pH: buffers, add acid/alkali from reservoir Maintain temp: cooling jacket/water bath 7 of 9 Testing - enzymes and starch Describe how to test of a product is contaminated with an enzyme Enzyme is a protein Burete test If enzyme present, solution turns lilac Describe how you would test to see if all starch is turned to sugar Benedicts/reducing sugars test Compare colour intesnity colorimeter/plot graph Continue until no further colour change/graph levels out 8 of 9 Explain advantages of using immobilised enzymes in Porduct is purer, enzymes dont have to be removed No enzymes lost, easy to reuse Process is cheaper, no removal of enzymes from product, less processes Enzyme stable in matrix, can be used at higher temps and more extreme pHs 9 of 9
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