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  • Created by: Primrose
  • Created on: 21-03-15 14:42

Light Microscope

  • Uses a number of lenses to veiw image
  • Light passes from bulb then through condenser lens through to specimen
  • Has 4 objective lenses to veiw specimen at different magnifications, x4, x10, x40 and x100


  • Has maximum magnification of x1500
  • Specimen such as living organism can be veiwed


  • Resolution of 200nm
  • If two objects are close together they are seen as one object
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Electron Microscopes

Consists of transmission electron microscope and scanning electron microscope

Transmission of electron microscope (TEM)

  • Produces 2D image
  • Has magnification of x500 000
  • Electons pass through dense part of samples less easier, giving contrast

Scanning electron Microscope (SEM)

  • Produces 3D image
  • Has maginfication of x100 000
  • Electrons do not pass through the specimen, they bounce off the sample
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Advantages and Disadvantages of Electron microscop


  • Maximum resolution of 0.2nm
  • Can be used to see inside cells


  • Samples have to be placed in vacuum as electron beams are deflected by the molecules in the air
  • Expensive
  • Require high skill to use
  • Final image is always black and white, color can be added with the use of specialised computer software
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