-Tissue is cut into pieces and put in a
COLD (to reduce enzyme activity which breaks down organelles),
BUFFERED (to maintain a constant pH),
ISOTONIC (to prevent organelles bursting/shrinking from osmotic gain/loss) SOLUTION.
-The tissue is HOMOGENISED (blended).
-The homogenate is FILTERED to REMOVE DEBRIS.
-Filtrate is CENTRIFUGED at a low speed.
-SUPERNATENT is analysed for content, then is decanted and re-centrifused at higher speeds until desired organelle is separeted.
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