Water mainly moves up the stem by Cohesion Tension, caused by Transpiration - the evaporation of water through the stomata of the leaves, which puts tension on the xylem, creating negative pressure, pulling more water up.
So, water moves up the xylem by pulling each molecule in a continuous, unbroken column.
Evidence:
1. Diameters of tree trunks - the diameter of trees is smaller in the day, when there is sunlight so stomata open to allow in CO2 for photosynthesis, meaning chance for more transpiration putting tension on the xylem, slimming the trunk.
2. If the xylem vessel is broken and air enters, then water is no longer pulled up, suggesting that the water is pulling each other up in an unbroken column.
3. If the xylem vessel is broken, the water does not leak out, suggesting the xylem is under tension rather than pressure.
Water also moves up the stem by capillary movement by adhesion - molecules stick to the walls and creep up that way.
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