Making Insulin

Making Insulin for GCSE

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  • Created by: Emily
  • Created on: 10-12-12 20:21

Stage 1

DNA removed from human cell.

Human insulin gene 'cut out' of DNA using restriction enzyme. 

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Stage 2

An enzyme cuts open bacterial plasmid. 

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Stage 3

Ligase enzyme joins gene to cut ends of plasmid.

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Stage 4

Plasmid with human gene is re-inserted into bacterium.

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Stage 5

Bacterium grows and reproduces quickly; each bacterial cell produces insulin using the human insulin gene. 

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Stage 6

In the correct conditions, the bacteria produce large quantities of human insulin. 

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