Magnification and calibration calculations

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Magnification and calibration


  • millimetre to micrometre = X1000
  • micrometre to nanometre = X1000
  • nanometre to micrometre = /1000
  • micrometre to millimetre = /1000

If you need to measure a structure as it would appear under a light microscope with an eyepiece graticule, you will be told the value of each of the divisions on the graticule.

                               Total size

Number of divisions        X           Value of each divisions

The following is the calculation for magnification:

                      Image size

Actual size             X         Magnification

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