Many genes are too long to be sequenced using the Sanger method all in one go.
Therefore they must be cut up using restriction enzymes, with the smaller fragments sequenced.
These smaller sequences must be pieced back together so that the whole sequence can be read. This is done using restriction mapping:
- Different restriction enzymes are used to cut labelled DNA into fragments.
- DNA fragments separated via electrophoresis.
- Size of fragments are used to determine the relative locations of the cut sites
- A restriction map of the DNA is produced - a diagram of the piece of DNA showing the different cut sites - recognition sites of the enzymes.
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