1. Carbon dioxide from the atmosphere diffuses into the leaf through the stomata and dissolves in water around the walls of mesophyll cells. It then diffuses into the stroma of the chloroplasts.
2. The carbon dioxide reacts with the 5-C compound RuBP, and this reaction is catalysed by an enzyme called rubisco.
3. The 6-C molecule produced is unstable, so the reaction instead produces two molecules of the 3-C GP.
4. NADPH is used to reduce the GP to TP, using energy supplied by ATP.
5. NADP and ADP + Pi are reformed in this reaction, and return to the light-dependent reaction.
6. Some molecules of TP are converted to organic substances such as starch, cellulose, lipids, glucose, amino acids, and nucleotides.
7. Most TP molecules are used to regenerate RuBP using ATP from the light-dependent reaction.
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