Immunological sandwich amplification.
Antiserum is harvested and placed in solution with the enzyme so that immune complexes form that remain soluble.
The molecular ratio of two molecules of IgG to three molecules of the enzyme.
Antiperoxidase antibodies are from the same species as that which has produced a primary antibody raised against tissue antigens
2 antibodies: one is directed against the tissue antigen. The other is directed against the peroxidase enzyme.
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