- one ATP is hydrolysed and the phosphate group attaches to glucose at carbon 6.
- Glucose 6-phosphate is changed to fructose 6-phosphate.
- Another ATP is hydrolysedand the phosphate group attaches to fructose 6-phosphate at carbon 1.
- The energy from ATP activates the hexose sugar, it is known as hexose 1,6 bisphosphate.
Splitting of hexose 1,6 bisphosphate:
- Hexose 1,6 bisphosphate is split into 2 molecules of triose phosphate
1 of 2
Oxidation of Triose Phosphate:
- two hydrogen atoms are removed from each triose phosphate molecule (involves dehydrogenase enzymes)
- NAD combines with the hydrogen to become reduced NAD
- two molecules of ATP are formed (substrate-level phosphorylation)
Conversion of Triose Phosphate to Pyruvate
- Triose phosphate is converted to an intermediate compound, then to pyruvate.
- two molecules of ATP are formed (substrate level phosphorylation)
net gain of 2ATP (total 4 ATP) , 2 reduced NAD, 2 pyruvate.
2 of 2